Little information is available regarding rearing and spawning Sonora Suckers in captivity. We are collaborating with UA and USU aquaculturists, AZGFD staff and the Salt River Project (SRP) to hold a captive population of Sonora Suckers from the Salt-Verde Rivers to investigate means to rear and spawn them in captivity. We attempted to rear the fish in both circular tanks and artificial streams with flow. Food and temperature were optimized for successful housing of the fish. Artificial streams provided the most successful rearing conditions. We attempted to induce natural spawning with manipulation of temperature, flow and photoperiod in the artificial streams. Spawning requirements of Sonora sucker previously obtained from the field were consulted to design treatments in the laboratory. The artificial streams were managed to induce natural spawning and egg survival. To date, natural inducement has been unsuccessful for inducing spawning. We also used hormonal injections involving Ovaprim and hand stripping of eggs and milt and fertilization in vitro. One hand stripping resulted in egg and milt expression, but no eggs were viable. Determination of appropriate environmental captive rearing and spawning conditions, sometimes after a period of initial mortality, has been essential to captively breed other Southwestern native fishes. We plan to continue treatments, and results of this work will be prepared for a thesis and publications.